Webb1 dec. 2007 · pRS41H are suitable as general t ransformat ion vectors and are parti cularl y usef ul for gene rating forced heter okaryons i n both fungal species. Acknow ledgements Webb5 feb. 2024 · Europe PMC is an archive of life sciences journal literature.
pRS41H-启动子酿酒酵母表达质粒--性能参数,报价/价格,图片_生 …
WebbpRS41H containing the hphNT1 gene was adopted as the template in a polymerase chain reaction (PCR) to amplify the hphNT1 open-reading frame flanked by regions homol-ogous to URA3 DNA sequences in pSH47. The plasmid Table 1 Primers used in this study Name Sequence(5′→3′) SPT15 F TCGAGTGCTAGCAAAATGGCCGATGAGGAA CGTTTAAAGG Webb28 nov. 2024 · Similarly, the amplified Δ9FAD fragment was inserted into a high copy plasmid pRS41H harbouring a quite strong promoter TEF1. Recombinant plasmid was … rsw board exam
Plasmid Bank
WebbYeast plasmids pRS41N and pRS41H that confer clonNAT and hygB resistance, respectively, to N. crassa and C. parasitica . Unique restriction sites in the multiple … WebbpRS41H are suitable as general transformation vectors and are particularly useful for generating forced heterokaryons in both fungal species. Acknowledgements. This work … Webb30 nov. 2010 · For overexpression of HAP4, a RPS2 (promotor)–HAP4 (overexpressed gene)–CYC1 (terminator) construct was cloned into the pRS41H plasmid. Liquid cultivations were carried out in minimal medium batch cultures as described by Blank and Sauer (2004) with 10 g/l glucose or galactose. rsw bottle hanger