WebEfficient mobility is dependent on both the RT activity of the aI2-encoded protein and a separate function, a putative DNA endonuclease, that is associated with the Zn 2+ finger-like region of the intron ... Our results demonstrate that the yeast group II intron aI2 is a retroelement that uses novel mobility mechanisms. Reprints and Corporate ... Webgroup I intron was reported to be essential for DNA endonuclease activity of these proteins. In other proteins, the EXlHH-HX3H domain is hypothesized to possess DNase activity as well. Pre- sumably, this activity promotes movement (rearrangement) of group I and group I1 introns encoding the EX,HH-HX3H do- main and other gene targets.

I-PpoI (Intron-Encoded Endonuclease) - Promega

WebMar 3, 2014 · Representative structures of homing endonuclease families and subfamilies. Top: three separate types of catalytic nuclease domains (GIY-YIG, HNH and EDxHD) … WebAll lanes : Anti-Intron-encoded endonuclease I-SceI antibody (ab216263) at 1/2000 dilution Lane 1 : Non-transfected 293T cells lysate Lane 2 : 293T cells transfected with … encore on demand movies

FOR THE RECORD Self-splicing group I I1 DNA a

WebMar 7, 2011 · The absence of an intron-coded ORF could be explained by the homing endonuclease being encoded by a gene present elsewhere ... RP Bonocora, DA Shub, A novel group I intron-encoded endonuclease specific for the anticodon region of tRNA(fMet) genes. Mol Microbiol 39, 1299–1306 (2001). Crossref. PubMed. Google … WebFunction. Mitochondrial DNA endonuclease involved in intron homing. It introduces a specific double-strand break in the DNA of the 21S rRNA gene and thus mediates the … Webgroup I intron was reported to be essential for DNA endonuclease activity of these proteins. In other proteins, the EXlHH-HX3H domain is hypothesized to possess DNase … dr bucktowarsing nephrology