WebFeb 20, 2024 · The fresh ascending aortic wall tissues were washed at least three times with Hanks Balanced Salt Solution (HBSS) (14025092, Thermo Fisher Scientific, Waltham, MA, USA) to wash away the blood. The adipose tissue attached to the adventitia was removed by blunt separation using forceps. Single cell preparation was performed on ice to … WebJul 21, 2024 · Culture medium was removed and both chambers rinsed gently with warm Hanks Balanced salt Solution (HBSS, catalogue number 14025092, Life Technologies, New Zealand). ... HBSS was then added to the apical (0.5 mL) and basolateral (1.5 mL) chambers and cells were allowed to equilibrate for 30 min. Then 10 μL of sodium …
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WebSep 3, 2024 · Each well were then rinsed with 0.5 mL Hank’s balanced salt solution (HBSS; Thermo Fisher Scientific, LLC; Grand Island, NY, USA; CAT: 14025092) to remove damaged associated molecular proteins. Afterward, 1 mL of treated or nontreated FGM was added into each well and returned to the incubator. Cells were imaged using Leica DMi1 … WebFeb 3, 2024 · experiments, Hank’s buffered salt solution (HBSS, 14025092) was pu rchased from Thermo . Fisher. Cells were transfected with Mito-RFP, Mito-GFP, mOrange-SKL, or Plin5 construct s . swallowing process
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WebSep 25, 2024 · Blocking GADD34 activity during starvation (HBSS + salubrinal versus HBSS) resulted in a significant (one-way ANOVA post hoc test, P < 0.1) decrease in the synthesis of the very proteins belonging to the endolysosomal system, including TFEB direct targets, as reported in Fig. 4 (A and B). WebOct 3, 2024 · Briefly, the cell-scaffold constructs (5x10 4 cells/scaffold) were removed from the culture plates at different days of incubation, washed with Hank’s balanced salt solution (HBSS; 14025092, Gibco). Then, the … http://www.dentalearner.com/page/2 skills for care wellbeing support