WebPA Janelia Fluor® 549, SE (PA-JF549-NHS) is a bright photoactivatable fluorophore of JF549,SE (JF549,NHS). JF549,SE (JF549,NHS) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm … WebThe synthesis and anion binding properties of a new family of fluorescent halogen bonding (XB) macrocyclic halo-imidazolium receptors are described. The receptors contain …
Quantification of very low-abundant proteins in bacteria …
WebHALO has been builders’, contractors’ and DIYers’ most trusted brand of residential downlighting for over 65 years. Learn about our latest HALO Canless products, hear … WebJun 21, 2024 · We stained the EWS/FLI1-Halo knock-in A673 cells with two fluorescent ligands (40, 41): ... Snapshots of an SPT movie imaging endogenous EWS/FLI1-Halo labeled with two Halo ligands, JF549 (200 nM) and PA-JF646 (20 nM). We imaged the EWS/FLI1-Halo hubs in the JF549 channel (green) and tracked individual EWS/FLI1 … new olympus pen camera
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WebAug 31, 2015 · dCas9 Fluorescent Labeling. For Halo domain labeling, fluorescent Halo ligands (JF549 and JF646) were mixed in protein samples at ratio of 8:1 and reacted at room temperature for 30 min followed by incubation at 4 °C overnight. The dCas9-Halo with an aldehyde tag was fluorescently labeled with Cy5 hydrazide (GE Healthcare) as … WebNov 18, 2014 · We report here the generation of a fluorescent protein (FP)-based dual molecular beacon (MB) system for nucleic acid detection. Halo-tag mediated conjugation … WebPromega now offers select Janelia Fluor® HaloTag® Ligands in a new format to support an enhanced workflow with less waste. The lyophilized format provides the following benefits: Improved workflow through direct … new olympus omd