2024 Atkat1

Atkat1

Author: gbte

August undefined, 2024

WebApr 10, 2010 · Gene-specific oligonucleotides were used to amplify the transcripts of AtCAMTA1, AtKAT1, AtAVP1 and AtCAMTA3. The amplification of the PCR products was measured by gel electrophoresis. For each gene, different numbers of PCR cycles were performed (as shown in Fig. 2 b and c). WebGlycine residues in potassium channel-like selectivity filters determine potassium selectivity in four-loop-per-subunit HKT transporters from plants

Protein–Protein Interactions between Sucrose Transporters of …

WebJan 1, 2024 · AtKAT1 and AtKAT2 form a heterotetramer and are involved in the regulation of guard cell movement. The hyperpolarization of the guard cell membrane by H + … WebAug 1, 2008 · Given that AtKAT1-mediated K + uptake did not show saturation in the substrate range measured, we were unable to estimate a K m for AtKAT1. To establish … troas pera

Planteome Curate - Planteome annotation page

WebMar 18, 2003 · A series of NubG-fusion proteins including membrane proteins of different compartments such as a the plasma membrane potassium channel AtKAT1 [ 25 ], the plasma membrane monosaccharide transporter AtSTP1 [ 26 ], a SEC12 homolog (AtStl2p) as an ER membrane protein [ 27 ], and orthologs of the soluble, but also partially … WebATKAT1, KAT1, POTASSIUM CHANNEL IN ARABIDOPSIS THALIANA 1 Description Encodes a potassium channel protein (KAT1). ABA triggers KAT1 endocytosis both in epidermal cells as well as guard cells. Upon removal of ABA, KAT1 is recycled back to the plasma membrane. KAT1 is localized within 0.5?0.6 μm diameter microdomains at the … WebFirst, we compared the amino acid sequences of OsKAT2 versus AtKAT1 and found that the 235th and 285th threonine of OsKAT2 corresponded to the 256th and 306th threonine of … troas history

AtCHX13 Is a Plasma Membrane K+ Transporter - PMC

WebJun 1, 2002 · The Arabidopsis AtKAT1 potassium channel and the AtSTP1 hexose transporter were expressed as NubG fusions and used as controls for unspecific interactions with other plasma membrane proteins. WBP1 corresponds to the weak yeast w heat germ agglutinin b inding p rotein promoter, and ADH1 to the a lcohol d e h ydrogenase promoter. WebUptake in AtKAT1-expressing yeast did not show saturation up to 5 mM K + , indicating a lower affinity for K + (Fig. 2C). After subtracting the K + uptake rate from vector controls, reciprocal... troas name meaningWebPlanteome annotation page/> Annotation: KAT1 locus:2170468 AT5G46240 AT5G46240 KAT1 AtKAT1 potassium channel in Arabidopsis thaliana 1 MPL12.2 MPL12_2 troas to philippi miles

"WebAug 13, 2013 · Here we report the characterization of two inward rectifying shaker-like potassium channels, OsKAT2 and OsKAT3, expressed in guard cell of rice plants. While OsKAT2 showed typical potassium channel activity, like that of Arabidopsis KAT1, OsKAT3 did not despite high sequence similarity between the two channel proteins. " - Atkat1

Atkat1

OsKAT2 is the prevailing functional inward rectifier potassium channels ...

WebDec 11, 2013 · The Arabidopsis AtHKT1;1 and AtKAT1 were used as controls in the growth inhibition experiments (Hirsch et al. 1998). As shown in the left panel of Figure 7 A, the … WebJun 22, 2007 · Growth was followed for 5 d. B, Growth of the K +-uptake-deficient yeast strain (CY162) transformed with the empty plasmid (pYES2), Arabidopsis KAT1 …

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WebNov 23, 2014 · A number of cyclic nucleotide gated channel (CNGC) genes have been identified in plant genomes, but their functions are mainly undefined. In this study, we … WebDec 9, 2024 · Therefore, the impact of phosphorylation on the regulation of these two transporters, could (1) reduce NO 3- influx as in its phosphorylated state AtNPF6.3 shifts to low capacity state and (2) increase K + influx, as …

WebWe identified mutation target amino acids, T235 and T285 of OsKAT2, corresponding T256 and T306 of AtKAT1, through sequence comparisons between AtKAT1 and OsKAT2. … WebSep 6, 2024 · Different studies in Arabidopsis suggested that it consist of voltage-dependent potassium ion channels known as AtKAT1 and are activated by external stimuli and are …

WebSep 23, 2024 · AtKAT1 (AT1G04710), a peroxisome-located gene, fused with red fluorescent protein (RFP) was used as a colocalization marker protein ( Fig. 2B ). Cotransforming nEYFP-IQM1 and cEYFP empty vector or nEYFP empty vector and cEYFP-CAT2/3 were set as negative controls. WebAtKAT1 plays roles as a major channel to uptake K + in guard cell when stomata open in dicot model plant Arabidopsis. In a recent publication, we isolated 3 KAT-like potassium …

WebOocytes injected with AtKAT1 cRNA showed typical inward K+ currents. Unlike AtKAT1, but similar to AtAKT2, OsAKT2 was characterized as a weakly rectifying K+ channel. The OsAKT2-mediated macroscopic currents also consisted of two kinetic components. One component was deﬁned as an instantaneous-activated

WebJan 1, 2013 · AtKAT1 plays roles as a major channel to uptake K+ in guard cell when stomata open in dicot model plant Arabidopsis. In a recent publication, we isolated 3 KAT … troast hearing aid ridgewood njWebMar 7, 2007 · In that study, transiently expressed AtKAT1 in tobacco was found in moderately detergent-resistant membrane fractions, possibly suggesting its location within lipid rafts. CONCLUSION. Future studies that delve deeper into the properties of the lipid raft-associated proteins should enable the discovery of what triggers the formation of … troast by lust en dustWebGene Model: AT5G46240.1. [. Help. ] Encodes a potassium channel protein (KAT1). ABA triggers KAT1 endocytosis both in epidermal cells as well as guard cells. Upon removal … troast hearing bloomfieldWebJan 1, 2024 · AtKAT1 and AtKAT2 form a heterotetramer and are involved in the regulation of guard cell movement. The hyperpolarization of the guard cell membrane by H + -ATPase activates the K + channels, such as the AtKAT1–AtKAT2 complex, resulting in opening of the stomata via increasing the K + influx. 28., 48., 49. troast surveyWebAug 13, 2013 · AtAKT1 and AtKAT1 were the first plant shaker-like potassium channel genes discovered. The AtKAT1 gene is expressed specifically in guard cells [6–8] and … troast auto body rockaway nj troast surveying associatesWebFig. 3 Homo-oligomerization of LeAMT1;1 and also AtKAT1 in split ubiquitin assay. Interactions among different N ub G, N ub WT, and C ub fusions were detected via growth assay on a synthetic minimal medium lacking adenine and histidine but supplemented with 0.075 m m methionine. The empty C ub vector was used as a negative control. troast hearing ridgewood nj